Detection of Carbapenem-resistant Enterobacteriaceae by Real-Time PCR and Assessment of Hospitalization Related Risk Factors Associated with their Fecal Carriage: A Case-control Study

Opportunistic behavior of commensal bacteria during severe infection, especially the intestine is always considered as a predisposing threat for severely ill patient admitted to hospitals. A descriptive-analytical case-control study was carried out detect prevalence and risk factor analysis fecal carriage Carbapenem-resistant Enterobacteriaceae. Patients having Enterobacteriaceae were cases patients who without taken control in proportion 1:4. (CRE) confirmed by both conventional well molecular methods. Methods such Combined Disk Test, mCIMtest, RAPIDEC CarbaNP Test used phenotypic identification CRE, Whereas Real-Time (RT)-PCR detection bla-gene encoded CRE (blaNDM1, blaVIM, blaKPC, blaIMP, blaOXA48). All belonging medicine wards included study. we screened 436 found 160 suitable our study, which 32 (20%) 128 (80%) controls. We total number 25 genes 16 isolates, where NDM 1 identified maximum numbers followed KPC &VIM. Standard statistical analyses chi-squire odd ratios conducted determine different variables. Stepwise multiple logistic regressions out, found, that transfer from other ward, use nebulizer intravenous catheter, antibiotics still statistically significant. Implementation Multi-modal colonization prevention need present situation throughout world.